US 8,411,358 B2Granted

Microscope apparatus

AssigneeOlympus Corporation

InventorYuichiro Matsuo

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Abstract

An arbitrary entire region of a specimen, or a plurality of individual regions, is simultaneously stimulated without a time lag, or a strong stimulus is applied to an arbitrary region of a specimen. The invention provides a microscope apparatus including a first stimulation optical system having a galvanometer mirror that scans a specimen with first stimulus light, which applies an optical stimulus to a specimen, on the specimen; a second stimulation optical system which has a plurality of two-dimensionally arrayed movable mirrors and which switches the angle of each movable mirror so that second stimulus light, which applies an optical stimulus to the specimen, can be selectively deflected towards the specimen; and a dichroic mirror that combines a light path of the first stimulation optical system and a light path of the second stimulation optical system.

Claims11

Dependent claims

A microscope apparatus comprising:

a first stimulation optical system including a galvanometer mirror that scans first stimulus light on a specimen to apply optical stimulus to the specimen;
a second stimulation optical system including a spatial light modulator that selectively switches an irradiation position of second stimulus light on the specimen to apply optical stimulus to the specimen;
a stimulation light-path combining section that combines a light path of the first stimulation optical system and a light path of the second stimulation optical system;
an observation optical system which acquires an image of the specimen, the observation optical system including a light source that illuminates the specimen, a scanning unit that scans illumination light on the specimen, and a photoelectric conversion device that receives light emitted from the specimen;
and
a whole-light path combining section that combines a light path of the observation optical system with the light paths of the first stimulation optical system and the second stimulation optical system combined by the stimulation light-path combining section.

3 more elements

Dependencies are inferred from the claim text.