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US 8,410,428 B2Granted
Methods for detecting dihydrotestosterone by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsAmit Ghoshal, Nigel J. Clarke, Richard Reitz
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Abstract
Provided are methods for determining the amount of dihydrotestosterone (DHT) in a sample using mass spectrometry. The methods generally involve ionizing DHT in a sample and detecting and quantifying the amount of the ion to determine the amount of DHT in the sample.
Claims12
A method for determining if a body fluid sample from a human patient contains an abnormal amount of dihydrotestosterone (DHT), said method comprising:
- a. ionizing underivatized DHT, purified by solid phase extraction (SPE) from the human body fluid sample, under conditions suitable to produce one or more ions detectable by mass spectrometry;
- b. determining the amount of one or more ions by mass spectrometry;
- c. using the amount of the one or more determined ions to calculate the amount of DHT in the body fluid sample;
- and
- d. comparing the determined amount of DHT in the body fluid sample with a reference range of the normal amount of DHT in the same body fluid.
2 more elements
Dependencies are inferred from the claim text.