Devices and methods for coupling mass spectrometry devices with chromatography systems
AssigneeAgilent Technologies, Inc.
InventorsCan C. Ozbal, Maxine Jonas, William A. LaMarr, Eduard Vernikov
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Abstract
One embodiment of the invention provides a method of preparing an eluted sample containing salts or buffers from a liquid chromatography device for analysis by a mass spectrometry device. The method includes: continuously providing a non-polar solvent to the mass spectrometry device; receiving the eluted sample from the liquid chromatography device; flowing the eluted sample over a solid phase extraction column; flowing the non-polar solvent over the solid phase extraction column; and presenting non-polar solvent and the eluted sample to the mass spectrometry device.
Claims19
A method comprising:
- continuously providing a non-polar solvent to a mass spectrometry device;
- applying a liquid sample to a first end of a solid phase extraction column;
- flowing the liquid sample over the solid phase extraction column in a first direction;
- flowing the non-polar solvent over the solid phase extraction column in a second direction;
- and
- presenting the non-polar solvent and the eluted sample received from the first end of the solid phase extraction column to the mass spectrometry device.
A sample injection system for coupling a liquid chromatography device with a mass spectrometry device, the system comprising:
- a sample injection valve having:
- i. a first position that allows sample from the liquid chromatography device to pass through the sample injection system, and
- ii. a second position that loads sample from the liquid chromatography device onto a sample supply loop;
- and
- a column control valve configured to facilitate a continuous flow of an elution solvent to a sample analyzer, the column control valve having:
- i. a first position that simultaneously delivers the fluidic sample from the sample supply loop to a solid phase extraction column in a first direction and delivers an elution solvent to the sample analyzer, and
- ii. a second position that flows the elution solvent over the solid phase extraction column in a second direction to deliver the fluidic sample and the elution solvent to the sample analyzer.
A method of preparing an eluted sample from a liquid chromatography device for analysis by a mass spectrometry device, the method comprising:
- continuously providing a polar solvent to the mass spectrometry device;
- receiving the eluted sample from the liquid chromatography device;
- flowing the eluted sample over a HILIC column in a first direction;
- flowing the polar solvent over the HILIC column in a second direction;
- and
- presenting the polar solvent and the eluted sample to the mass spectrometry device.
Dependencies are inferred from the claim text.