Fluorescence microscopy imaging system

A fluorescence microscopy imaging system, for detecting a fluorescence signal of a sample, comprising:

• a module for detecting fluorescence, having a fluorescence excitation light source generator, an optical assemble module of fluorescence detection, and a fluorescence detector, wherein the fluorescence excitation light source generator is capable of generating an excitation light beam having a first wavelength, the excitation light beam is guided by the optical assemble module of fluorescence detection along a light path to pass through an objective and focused on the sample so as to excite the sample to emit a fluorescence light, and then the excited fluorescence light of the sample is guided by the optical assemble module of fluorescence detection along the light path to the fluorescence detector;
• a module for focusing control, having a servo light source generator, a dichroic optical element, an optical assemble module of servo focus, an actuator, a servo light beam reflecting film, and a focusing detection device, wherein the servo light source generator is capable of generating a servo light beam having a second wavelength, the servo light beam reflecting film is disposed on an observation plane of the sample, the dichroic optical element is disposed at an area of intersection of the optical assemble module of servo focus and the light path, and is used to couple the servo light beam to the light path, such that the servo light beam passes through the objective and reaches the servo light beam reflecting film, and then guides a return beam signal reflected back from the servo light beam reflecting film along a servo light path to the focusing detection device, the focusing detection device controls the actuator to move along an optical axis of the light path according to the return beam, and the actuator is used to move the objective for focusing;
• and
• an optical fiber, for receiving the fluorescence light excited after the excitation light beam focuses on the sample through the objective, and then introducing the fluorescence light into the fluorescence detector.

A fluorescence microscopy imaging system, for detecting a fluorescence signal of a sample, comprising:

• a module for detecting fluorescence, having a fluorescence excitation light source generator, an optical assemble module of fluorescence detection, and a fluorescence detector, wherein the fluorescence excitation light source generator is capable of generating an excitation light beam having a first wavelength, the excitation light beam is guided by the optical assemble module of fluorescence detection along a light path to pass through an objective and be focused on the sample so as to excite the sample to emit a fluorescence light, and then the excited fluorescence light of the sample is guided by the optical assemble module of fluorescence detection along the light path to the fluorescence detector;
• and
• a module for focusing control, having a servo light source generator, a dichroic optical element, an optical assemble module of servo focus, an actuator, a servo light beam reflecting film, and a focusing detection device, wherein the servo light source generator is capable of generating a servo light beam having a second wavelength, the servo light beam reflecting film is disposed on an observation plane of the sample, the dichroic optical element is disposed at an area of intersection of the optical assemble module of servo focus and the light path, and is used to couple the servo light beam to the light path, such that the servo light beam passes through the objective and reaches the servo light beam reflecting film, and then guides a return beam signal reflected back from the servo light beam reflecting film along a servo light path to the focusing detection device, the focusing detection device controls the actuator to move along an optical axis of the light path according to the return beam, and the actuator is used to move the objective for focusing;
• wherein the observation plane is a cross section at a location of intersection of the light path and the sample, the servo light beam reflecting film is disposed at a light transmissible element located on the observation plane, the servo light beam reflecting film is disposed on any one of an upper surface of a cover glass covering the sample, a lower surface of a cover glass covers the sample, and an upper surface of a slide glass carries the sample.