Plant tubby-like proteins
AssigneeAcademia Sinica
InventorsJei-Fu Shaw, Chia-ping Lai, Po-Hong Chen
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Abstract
An isolated polypeptide containing an amino acid sequence at least 70% identical to a Tubby-like protein (SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11), and an isolated nucleic acid encoding the polypeptide. Disclosed is an isolated nucleic acid that, under stringent conditions, hybridizes to a probe containing one of SEQ ID NOs:1-11; or its complementary sequence. Also disclosed are (1) a transformed cell or a transgenic plant containing such a nucleic acid and (2) a transformed cell or a transgenic plant having a reduced level(s) of one or more of the Tubby-like proteins. Also within the scope of the invention are methods for making the transformed cells or transgenic plants.
Claims3
An Arabidopsis plant cell transformed with a DNA construct that expresses an interfering RNA specific to an mRNA encoding Arabidopsis tubby-like protein 11 (AtTLP11), wherein said DNA construct comprises a promoter operably linked to a first DNA strand comprising the nucleotide sequence from position 851 to position 1140 of SEQ ID NO:
- 22 and a second DNA strand that is fully complementary to the first DNA strand, wherein the first and second DNA strands are arranged in a 5′ to 3′ direction with respect to each other, wherein the plant cell expresses said interfering RNA which is a double-stranded hairpin RNA encoded by said DNA construct, and wherein said expression results in reduced expression of AtTLP 11 protein in said transformed plant cell as compared to an untransformed Arabidopsis plant cell lacking said DNA construct.
Dependencies are inferred from the claim text.