Transgenic plants with enhanced agronomic traits
AssigneeMonsanto Technology LLC
InventorLinda L. Lutfiyya
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Abstract
This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
Claims15
A transgenic plant cell having recombinant DNA in its chromosomal DNA wherein said recombinant DNA comprises (a) a promoter that is functional in a plant cell and that is operably linked to a polynucleotide that encodes a protein having an amino acid sequence having at least 95% identity over at least 95% of the length of SEQ NO:13 and having trehalose phosphate synthase activity and (b) a promoter that is functional in a plant cell and that is operably linked to a polynucleotide that encodes a protein having an amino acid sequence having at least 95% identity over at least 95% of the length of SEQ ID NO:14 and having trehalose phosphatase activity.
A method of producing hybrid corn seed, said method comprising:
- (a) acquiring hybrid corn seed from an herbicide tolerant corn plant, wherein the chromosomal DNA of said corn seed comprises recombinant DNA comprising:
- (i) a promoter that is functional in a plant cell operably linked to a polynucleotide that encodes a protein having an amino acid sequence having at least 95% identity over at least 95% of the length of SEQ ID NO:
- 13 and having trehalose phosphate synthase activity;
- and
- (ii) a promoter that is functional in a plant cell operably linked to a polynucleotide that encodes a protein having an amino acid sequence having at least 95% identity over at least 95% of the length of SEQ ID NO:
- 14 and having trehalose synthase activity;
- (b) producing corn plants from said hybrid corn seed, wherein a fraction of the plants produced from said hybrid corn seed are homozygous for said recombinant DNA, a fraction of the plants produced from said hybrid corn seed are hemizygous for said recombinant DNA, and a fraction of the plants produced from said hybrid corn seed has none of said recombinant DNA;
- (c) selecting corn plants which are homozygous and hemizygous for said recombinant DNA by treating with a herbicide;
- (d) collecting seed from herbicide-treated-surviving corn plants and planting said seed to produce further progeny corn plants;
- (e) repeating steps (c) and (d) at least once to produce an inbred corn line;
- and
- (f) crossing said inbred corn line with a second corn line to produce hybrid seed.
Dependencies are inferred from the claim text.