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AssigneeMonsanto Technology LLC
InventorsEdwards Allen, William P. Donovan, Gregory R. Heck, James K. Roberts, Virginia Ursin, Yuanji Zhang
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This invention provides a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest, recombinant DNA constructs for expression of at least one invertebrate miRNA in planta and methods of use thereof, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention.
A non-natural plant having resistance to an invertebrate pest that ingests RNA from said plant, wherein
A non-natural transgenic plant cell having in its genome a recombinant DNA construct that comprises a promoter functional in a plant cell and that is transcribed in said non-natural transgenic plant cell to a recombinant miRNA precursor, wherein said recombinant miRNA precursor comprises a single strand of RNA that folds into a secondary structure of an invertebrate a miRNA precursor identified from an invertebrate genome and that comprises at least one stem-loop that is processed to a mature miRNA, and wherein said mature miRNA suppresses expression of at least one target gene of an invertebrate or of a symbiont associated with said invertebrate.
Dependencies are inferred from the claim text.