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US 8,410,333 B2Granted
Humanized immunoglobulin loci
AssigneeTherapeutic Human Polyclonals, Inc.
InventorsRoland Buelow, Wim van Schooten, Josef Platzer
Life of this patent
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Abstract
The present invention concerns methods and means to produce humanized antibodies from transgenic non-human animals. The invention specifically relates to novel immunoglobulin heavy and light chain constructs, recombination and transgenic vectors useful in making transgenic non-human animals expressing humanized antibodies, transgenic animals, and humanized immunoglobulin preparations.
Claims2
A transgenic rabbit, comprising a humanized immunoglobulin locus comprising multiple Ig gene segments wherein
- (a) at least one of said gene segments is a human Ig gene segment comprising two or more identical or different units consisting of, from 5′ to 3′ direction, a 5′ nucleotide sequence, a human immunoglobulin heavy or light chain V gene segment, and a 3′ nucleotide sequence, wherein said 5′ nucleotide sequence and said 3′ nucleotide sequence have the nucleotide sequence of SEQ ID NO 35;
- (b) said gene segments are juxtaposed in an unrearranged, partially rearranged or fully rearranged configuration, and
- (c) said humanized Ig locus is capable of undergoing gene rearrangement, if necessary, and gene conversion and/or hypermutation, and producing a repertoire of humanized immunoglobulins in said transgenic rabbit.
Dependencies are inferred from the claim text.