Recombinant expression vector elements (REVES) for enhancing expression of recombinant proteins in host cells
AssigneeAbbVie Inc.
InventorsWendy R. Gion, Gerald R. Carson, Hong Gao, Yune Z. Kunes
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Abstract
Compositions and methods comprising recombinant expression vector elements (rEVEs) to enhance the level of expression of recombinant proteins are described. Other compositions and methods for lowering, substantially suppressing, or essentially silencing expression of a recombinant protein are also described.
Claims12
A yeast host cell comprising a recombinant vector, wherein the recombinant vector comprises a recombinant expression vector element (rEVE) polynucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- the nucleotide base sequence of SEQ ID NO:1, the nucleotide base sequence of SEQ ID NO:2, a sequence complementary to any foregoing sequence, an expression-enhancing portion of any foregoing sequence, and combinations thereof;
- and wherein the yeast host cell is selected from the group consisting of:
- Hansenula polymorpha, Kluyveromyces lactis, Pichia pastoris, Schizosaccharomyces pombe, and Yarrowia lipolytica.
A nematode host cell comprising a recombinant vector, wherein the recombinant vector comprises a recombinant expression vector element (rEVE) polynucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- the nucleotide base sequence of SEQ ID NO:1, the nucleotide base sequence of SEQ ID NO:2, a sequence complementary to any foregoing sequence, an expression-enhancing portion of any foregoing sequence, and combinations thereof;
- and wherein the nematode host cell is Caenorhabditis elegans.
A prokaryotic host cell comprising a recombinant vector, wherein the recombinant vector comprises a recombinant expression vector element (rEVE) polynucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- the nucleotide base sequence of SEQ ID NO:1, the nucleotide base sequence of SEQ ID NO:2, a sequence complementary to any foregoing sequence, an expression-enhancing portion of any foregoing sequence, and combinations thereof;
- and wherein the prokaryotic host cell is selected from the group consisting of:
- Escherichia coli, a serovar of Salmonella enterica, a Shigella species, Wollinella succinogenes, Proteus vulgaris, Proteus mirabilis, Edwardsiella tarda, Citrobacter freundii, a Pasteurella species, a Haemophilus species, a Pseudomonas species, a Bacillus species, a Staphyloccocus species, and a Streptococcus species.
A method of producing a recombinant protein of interest comprising:
- inserting into a host cell a recombinant expression vector wherein the recombinant expression vector comprises:
- one or more genes encoding the recombinant protein of interest;
- and
- a nucleotide base sequence selected from the group consisting of:
- the nucleotide base sequence of SEQ ID NO:1, the nucleotide base sequence of SEQ ID NO:2, a sequence complementary to any foregoing sequence, an expression-enhancing portion of any foregoing sequence, and combinations thereof;
- and
- culturing the host cell under conditions promoting expression of the recombinant protein of interest.
Dependencies are inferred from the claim text.