Dark quenchers for donor-acceptor energy transfer
AssigneeBiosearch Technologies, Inc.
InventorsRonald M. Cook, Matt Lyttle, Daren Dick
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Abstract
The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.
Claims15
A quencher of excited state energy having a formula which is a member selected from:
A quencher of excited state energy, having the formula:
- wherein
- X5 is —NR′R″,
- in which R′ and R″ are members independently selected from unsubstituted alkyl and substituted alkyl,
- with the proviso that at least one of R′ and R″ is selected such that it bears a reactive functional group.
A quencher of excited state energy, having the formula:
- wherein
- X5 is —NR′R″,
- in which R′ and R″ are members independently selected from unsubstituted alkyl and substituted alkyl,
- with the proviso that at least one of R′ and R″ is selected such that it bears a reactive functional group,
- wherein said reactive functional group is a member selected from carboxy, N-hydroxysuccinimide ester, N-hydroxybenztriazole ester, acid halide, acyl imidazole, thioester, p-nitrophenyl ester, alkyl ester, alkenyl ester, alkynyl ester, aromatic ester, and —NH2.
A quencher of excited state energy, having the formula:
- wherein X5 is
- wherein
- X1 is —CH3;
- X2 is —OP(OX3)N(X4)2,
- wherein X3 is cyanoethyl;
- and X4 is isopropyl;
- and
- p and q are numbers independently selected from 1 to 20, inclusive.
A quencher of excited state energy, having the formula:
- wherein
- X5 and X6 are each —NR′R″,
- in which R′ and R″ are members independently selected from unsubstituted alkyl and substituted alkyl,
- with the proviso that for at least one of X5 and X6 at least one of R′ and R″ is selected such that it bears a reactive functional group.
Dependencies are inferred from the claim text.