Stabilized activin IIB receptor polypeptides and uses thereof
AssigneeAtara Biotherapeutics, Inc.
InventorsJeonghoon Sun, Lei-Ting Tony Tam, Mark Leo Michaels, Thomas C. Boone, Rohini Deshpande, Yue-Sheng Li, Hq Han
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Abstract
The present invention provides stabilized activin IIB receptor polypeptides and proteins capable of binding and inhibiting the activities of activin A, myostatin, or GDF-11. The present invention also provides polynucleotides, vectors and host cells capable of producing the stabilized polypeptides and proteins. Compositions and methods for treating muscle-wasting diseases and metabolic disorders are also provided.
Claims15
An isolated protein comprising a stabilized activin IIB receptor polypeptide (svActRIIB) wherein said polypeptide is selected from the group consisting of:
- (a) a polypeptide consisting of the sequence set forth in SEQ ID NO:
- 2, except for a single amino acid substitution at position 28, and a single amino acid substitution at position 44, wherein the substitution at position 28 is selected from the group consisting of W and Y, and the substitution at position 44 is T;
- (b) a polypeptide consisting of the sequence set forth in amino acids 19 through 134 of SEQ ID NO:
- 2, except for a single amino acid substitution at position 28, and a single amino acid substitution at position 44, wherein the substitution at position 28 is selected from the group consisting of W and Y, and the substitution at position 44 is T;
- (c) a polypeptide consisting of the sequence set forth in amino acids 23 through 134 of SEQ ID NO:
- 2, except for a single amino acid substitution at position 28, and a single amino acid substitution at position 44, wherein the substitution at position 28 is selected from the group consisting of W and Y, and the substitution at position 44 is T;
- (d) a polypeptide consisting of the sequence set forth in amino acids 25 through 134 of SEQ ID NO:
- 2, except for a single amino acid substitution at position 28, and a single amino acid substitution at position 44, wherein the substitution at position 28 is selected from the group consisting of W and Y, and the substitution at position 44 is T;
- (e) a polypeptide of any one of (a) through (d), except for a single amino acid substitution at position 28, and a single amino acid substitution at position 44, wherein the substitution at position 28 is selected from the group consisting of W and Y, and the substitution at position 44 is T, wherein the polypeptide is capable of binding myostatin, activin A, or GDF-11.
An isolated protein comprising a stabilized activin IIB receptor polypeptide, wherein said polypeptide is selected from a polypeptide consisting of the sequence set forth in the group consisting of SEQ ID NO:
- 4, 6, 12 and 14.
Dependencies are inferred from the claim text.