Cartridge for automatic measurement and measuring method using it

A measuring method for a component to be measured contained in a sample, comprising:

• providing a cartridge comprising at least two or more lines of well groups arranged in parallel or providing plural cartridges each having at least one line of well groups, wherein each well group comprises a diluting well for diluting a predetermined amount of the sample to a desired dilution, and a reaction well in which the component to be measured contained in the sample and a substance specifically reacting therewith are allowed to react, wherein a diluting solution is filled in the diluting well of each well group in a predetermined amount to provide the desired dilution depending on a type of the component to be measured and then sealing the cartridge or plural cartridges using a cartridge seal or cartridge seals;
• piercing the cartridge seal or cartridge seals and dispensing the predetermined amount of sample in the diluting well of each well group already filled with the predetermined amount of diluting solution with a dispensing mechanism comprising multiple dispensing elements in a uniform operation to thereby dilute the sample to the desired dilution in each diluting well of each well group;
• reacting the component to be measured in the diluted sample with the substance specifically reacting therewith;
• and
• measuring an amount of a reaction product.