Methods and kits for the determination of the presence and quantity of vitamin D analogs in samples
AssigneeWaters Technologies Corporation
InventorsLisa Jane Calton, Kendon Stuart Graham
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Abstract
Embodiments of the present invention are directed to methods and kits for determining the presence or absence, and the amount if present, of vitamin D analogs in samples. One embodiment of the present method for detecting the presence or absence of vitamin D analogs in a sample comprises the steps of adding an effective amount of a multiple charge cationic agent to the sample to form a cationic treated sample. The effective amount of a multiple charge cationic agent enhances the signal from vitamin D analogs upon analysis by mass spectroscopy.
Claims17
A method for detecting the presence or absence of Vitamin D analogs in a sample comprising the steps of:
- adding an effective amount of a multiple charge cationic agent to said sample to form a cationic treated sample, said effective amount of said multiple charge cationic agent enhancing the signal from Vitamin D analogs upon further analysis by mass spectroscopy;
- subjecting said cationic treated sample to centrifugation to form a supernatant;
- separating proteins in said supernatant from said Vitamin D analogs by protein precipitation to form an analyte solution;
- placing said analyte solution on an analytical column and forming a eluent having said Vitamin D analogs separated from each other and non-Vitamin D compounds,
- placing said eluent in a mass spectrometer and detecting the presence or absence of Vitamin D analogs in the sample by mass spectra.
A kit for detecting the presence or absence of Vitamin D analogs in a sample comprising:
- an effective amount of a multiple charge cationic agent, a protein precipitating agent, and instructions for use of said multiple charge cationic agent in a method to detect the presence or absence of Vitamin D analogs;
- said method comprising adding said effective amount of a multiple charge cationic agent to said sample to form a cationic treated sample, said effective amount of the multiple charge cationic agent enhancing the signal from Vitamin D analogs upon further analysis by mass spectroscopy;
- centrifuging said treated sample to form a supernatant, separating protein in said supernatant from said Vitamin D analogs by protein precipitation with said protein precipitating agent to form an analyte solution, placing said analyte solution on an analytical column and forming an eluent having said Vitamin D analogs separated from each other and non-Vitamin D compounds, and placing said eluent in a mass spectrometer and detecting the presence or absence of Vitamin D analogs in the sample by mass spectra.
Dependencies are inferred from the claim text.