Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
AssigneeRegents of the University of Minnesota
InventorsCatherine M. Verfaillie, Miguel Angel Barajas Velez, Yves Pierre Heremans
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Abstract
The invention provides methods for differentiating non-embryonic multipotent stem cells along the pancreatic lineage. The present invention further provides non-embryonic multipotent stem cells and progeny derived therefrom to provide pancreatic cells to a subject.
Claims11
An in vitro method comprising contacting non-embryonic stem, non-germ, non-embryonic germ cells that can differentiate into at least two of ectodermal, endodermal and mesodermal cell types with agents consisting essentially of Activin A and an agent that inhibits sonic hedgehog (SHH) activity to yield cells having increased expression of Pdx-1.
An in vitro method to differentiate non-embryonic stem, non-germ, non-embryonic germ cells that can differentiate into at least two of ectodermal, endodermal and mesodermal cell types comprising the steps of:
- a) contacting the non-embryonic stem, non-germ and non-embryonic germ cells with factors consisting essentially of Activin A and an agent that inhibits sonic hedgehog activity, to produce cells with increased expression of Pdx-1;
- b) contacting the cells obtained from step a) with factors consisting essentially of EGF or HGF, to produce cells expressing Ngn3;
- and
- c) contacting the cells obtained from step b) with factors consisting essentially of one or both of nicotinamide or exendin4, to produce cells expressing insulin.
A composition comprising (1) factors consisting essentially of Activin A and an agent that inhibits sonic hedgehog and (2) non-embryonic stem, non-germ, non-embryonic germ cells that differentiate into at least two of ectodermal, endodermal and mesodermal cell types.
Dependencies are inferred from the claim text.