Recombinant adeno-associated virus production
AssigneeNationwide Children's Hospital, Inc.
InventorsKelly Reed Clark, Philip R. Johnson, Jr.
Life of this patent
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Abstract
The present invention relates to methods and materials for recombinant adeno-associated virus production. More particularly, the invention relates to use of recombinant adenovirus encoding adeno-associated virus protein in recombinant adeno-associated virus production methods.
Claims11
A recombinant E3-deleted Ad stably expressing AAV Rep proteins consisting of Rep 52 and Rep 40.
A method of generating a rAAV-producing cell comprising the step of infecting a cell with one or more recombinant adenoviruses and one or more recombinant vaccinia viruses, wherein a recombinant adenovirus provides a rAAV genome and AAV cap DNA, wherein a recombinant vaccinia virus provides rAAV rep 78 DNA, and wherein a recombinant adenovirus provides AAV Rep DNA consisting of AAV rep 52/40 DNA.
Dependencies are inferred from the claim text.