Hydroxynitrile lyase
AssigneeMitsubishi Rayon Co., Ltd.
InventorsYasuhisa Asano, Takanori Akiyama, Fujio Yu, Eiji Sato
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Abstract
An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
Claims29
An isolated or purified modified hydroxynitrile lyase
- that is at least 90% homologous to the wild-type hydroxynitrile lyase of Manihot escuela of SEQ ID NO:
- 1 or at least 90% homologous to the wild-type hydroxynitrile lyase of Hevea brasiliensis of SEQ ID NO:
- 102, and
- that has a substitution of at least one lysine residue of the wild-type hydroxynitrile lyase with another kind of amino acid residue.
Dependencies are inferred from the claim text.