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US 8,409,840 B2Granted
Recombinant preparation of selected bromelain fractions
AssigneeUrsapharm Arzneimittel GmbH
InventorsRolf Müller, Nora Luniak, Klaus Eschmann
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Abstract
The present invention pertains in general to Bromelain and particularly to the different active compounds contained in this complex mixture of proteins. The present invention provides recombinant expressed cysteine proteases, which are found in Bromelain. It has been found that the method for expression of the recombinant proteins is superior to the purification from Bromelain itself.
Claims9
A heterologously expressed, soluble, fragment of Bromelain protein having cysteine protease activity wherein the fragment comprises
- (a) at least 130 consecutive amino acids of the amino acid sequence of SEQ ID NO:1, and
- (b) the active site of the Bromelain protein, wherein the C-terminal propeptide from position 326 to position 359 of SEQ ID NO:1 is not present in said fragment.
Dependencies are inferred from the claim text.