Sensor proteins and assay methods
AssigneeLife Technologies Corporation
InventorsKurt Vogel, Rhonda Newman, Steven Riddle
Life of this patent
Estimating the term…
Abstract
The present invention relates to biosensors. In some embodiments, the biosensors are modified ligand binding molecules. In some embodiments, the modified ligand binding molecule is a phosphate binding protein (PBP). In some embodiments, the modified ligand binding molecules are labeled to be capable of RET, e.g., comprising a donor and acceptor moiety. In some embodiments of the invention, there is a detectable change in RET (e.g., FRET) when the modified ligand binding molecule binds and/or releases the ligand (e.g., phosphate). The invention also provides related methods, reactions and assays.
Claims20
A phosphate binding protein comprising a resonance energy transfer (RET) pair of moieties comprised of at least one donor moiety and at least one acceptor moiety, wherein the donor moiety comprises a lanthanide metal complex, wherein neither the donor nor acceptor moiety is a fluorescent protein, and wherein the phosphate binding protein is capable of binding inorganic phosphate and wherein the binding results in a change in RET.
Dependencies are inferred from the claim text.