Allelic ladder loci
AssigneeLife Technologies Corporation
InventorsRobert Green, Julio Mulero, Lori Hennessy, Robert Lagace, Chien-Wei Chang
Life of this patent
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Abstract
Disclosed are rare short tandem repeat (STR) alleles within the D10S1248 and D12S391 loci in humans. Provided are representative allelic ladders for each locus, methods and assays using these alleles and kits containing allelic ladders comprising these alleles for accurate genotyping and identification of a wide range of individuals.
Claims24
An allelic ladder comprising, in combination, an isolated plurality of nucleic acid molecules which are allelic variants of one or more polymorphic short tandem repeat loci, wherein the polymorphic short tandem repeat locus is at least one of D10S1248 comprising allele-7 and D12S391 comprising allele-13, wherein D10S1248 allele-7 is short tandem repeats consisting of the sequence of SEQ ID NO:
- 10 and wherein D12S391 allele-13 is not an imperfect, incomplete or variant repeat of SEQ ID NO:
- 11.
An allelic ladder mixture comprising an allelic ladder for one or more of the following loci, with lowest and highest allele designation as shown, wherein D10S1248 allele-7 is not an imperfect, incomplete or variant repeat of SEQ ID NO:
- 10 and wherein D12S391 allele-13 is not an imperfect, incomplete or variant repeat of SEQ ID NO:
- 11:
- Locus
- Low MW Allele
- High MW Allele
- D10S1248
- 7
- 19
- D12S391
- 13
- 27.
A kit for analyzing a short tandem repeat sequence from a nucleic acid sample, the kit comprising, an allelic ladder for detecting allele-7 of D10S1248, and a pair of amplification primers for producing an amplification product from the D10S1248 locus and an allelic ladder for detecting allele-13 of D12S391, and a pair of amplification primers for producing an amplification product from the D12S391 locus, wherein D10S1248 allele-7 is short tandem repeats consisting of the sequence of SEQ ID NO:
- 10 and wherein D12S391 allele-13 is not an imperfect, incomplete or variant repeat of SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.