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US 8,409,625 B2Granted
Conditioned decellularized native tissues for tissue restoration
AssigneeACell, Inc.
InventorStephen F. Badylak
Life of this patent
Estimating the term…
Abstract
The invention provides a composition conditioned for the remodeling, restoration, repair, or replacement of tissue within a host. The composition is conditioned by culturing cells on the matrix and/or by exposing the cultured cells or matrix to one or more stressors.
Claims15
A method for conditioning a decellularized composition, comprising:
- (i) exposing cultured epithelial cells to a stressor selected from the group consisting of a mechanical stimulus, an electrical stimulus, hypoxia, and hypercarbia to condition said epithelial cells;
- (ii) providing a naturally-occurring matrix comprising native epithelial cells and structural components of an extracellular matrix arranged in their native orientation obtained from an epithelial tissue of a mammal;
- (iii) decellularizing the naturally-occurring matrix to remove said native epithelial cells;
- then
- (iv) conditioning the decellularized matrix by culturing said conditioned epithelial cells on the decellularized matrix;
- followed by
- (v) decellularizing the conditioned matrix to remove the conditioned epithelial cells.
4 more elements
A method for manufacturing a decellularized conditioned composition for inducing restoration, remodeling, replacement or repair of a tissue in a patient, the method comprising:
- conditioning epithelial cells of interest by exposing the epithelial cells to at least one stressor selected from the group consisting of a mechanical stimulus, an electrical stimulus, hypoxia and hypercarbia;
- providing a naturally-occurring matrix obtained from an epithelial tissue of a mammal comprising structural components of an extracellular matrix arranged in their native orientation;
- then
- decellularizing the naturally-occurring matrix to remove native cells;
- then
- conditioning the decellularized matrix by culturing said conditioned epithelial cells on the decellularized matrix in vitro;
- followed by
- decellularizing the conditioned matrix to remove the conditioned epithelial cells thereby forming the decellularized conditioned composition that induces restoration, remodeling, replacement or repair of the tissue.
5 more elements
Dependencies are inferred from the claim text.