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US 8,409,588 B2Granted
Recombinant foot and mouth disease vaccine
OwnerMarkus Czub, Alfonso Clavijoinventors
Life of this patent
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Abstract
A foot and mouth disease virus (FMDV) vaccine and method for producing same is described wherein the N terminal portion of the FMDV polyprotein, encoding the four structural proteins, 1A, 1B, 1C, and 1D, are each separated by a non-FMDV protease, preferably a cellular protease, for example, furin. The expression system may be transformed into a cell expressing the non-FMDV protease and the resulting particles recovered for use as a vaccine.
Claims8
An expression system comprising
- a promoter operably linked to a nucleic acid molecule encoding a poly-protein, said polyprotein comprising:
- FMDV 1A protein furin protease recognition sequence-FMDV 1B protein furin protease recognition sequence-FMDV 1C protein furin protease recognition sequence-FMDV 1D protein, wherein the poly-protein lacks FMDV proteinasse 2A.
A method of producing a foot and mouth disease virus-like particle comprising:
- providing a host cell including an expression system comprising a promoter operably linked to a nucleic acid molecule encoding a poly-protein, said polyprotein comprising FMDV 1A protein furin protease recognition sequence-FMDV 1B protein furin protease recognition sequence-FMDV 1C protein furin protease recognition sequence-FMDV 1D protein, said host cell expressing furin protease;
- growing the host cell under conditions such that the poly-protein is produced, resolved into 1A, 1B, 1C and 1D by the furin protease and 1A, 1B, 1C and 1D assemble into virus-like particles;
- and
- recovering the virus-like particles.
1 more element
Dependencies are inferred from the claim text.