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US 8,409,583 B2Granted
Engineered versions of CgtB (β-1,3 galactosyltransferase) enzymes, with enhanced enzymatic properties
AssigneeNational Research Council of Canada
InventorsStephane Bernatchez, Michel Gilbert, Warren Wakarchuk
Life of this patent
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Abstract
CgtB proteins with enhanced beta 1,3-galactosaminyltransferase activity, nucleic acids that encode the CgtB proteins and methods for use of the CgtB proteins.
Claims12
A β-1,3-galactosyltransferase fusion polypeptide comprising
- i. a truncated CgtB polypeptide, wherein between 1 and 35 amino acids are removed from the C terminal end of the CgtB polypeptide, and
- ii. a maltose binding protein domain fused to the C-terminus of the CgtB polypeptide,
- wherein the β-1,3-galactosyltransferase polypeptide transfers a galactose moiety from a donor substrate to an acceptor substrate and wherein the truncated CgtB polypotide comprises an amino acid sequence with at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 and SEQ ID NO:
- 10.
1 more element
Dependencies are inferred from the claim text.