Anti-CD71 monoclonal antibodies and uses thereof for treating malignant tumor cells
AssigneesMablife, Institut National De La Sante Et De La Recherche Medicale (Inserm)
InventorsLaurence Boumsell, Jean Kadouche, Armand Bensussan
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Abstract
The present invention provides novel anti-CD71 monoclonal antibodies, in particular mouse-human chimeric anti-CD71 monoclonal antibodies, advantageously associated to effector cells for triggering ADCC mechanisms. Anti-CD71 antibodies, as well as pharmaceutical compositions containing them, are useful for inhibiting proliferation and/or killing malignant tumour cells, especially metastatic cutaneous and uveal melanoma cells.
Claims5
A method for inhibiting proliferation or killing CD71 positive malignant tumor cells, comprising providing to a patient in need thereof
- an anti-CD71 monoclonal antibody or an antigen-binding fragment thereof, wherein said anti-CD71 monoclonal antibody comprises
- (a) at least one heavy chain variable region comprising the amino acid sequence of SEQ ID No. 1 and at least one light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID No. 2 and SEQ ID No. 11;
- or
- (b) at least one heavy chain variable region encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID No. 3 and at least one light chain variable region encoded by a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID No. 4 and SEQ ID No. 12
- under conditions and in an amount sufficient for the binding of said anti-CD71 monoclonal antibody to the malignant tumor cells, thereby causing inhibition of proliferation or killing of the malignant tumor cells.
A method for producing a chimeric antibody that binds CD71, comprising:
- a) transforming a suitable host cell with at least one expression vector comprising two or more of the nucleotide sequences depicted in SEQ ID Nos. 3, 4, and 12;
- b) culturing said host cell under conditions favoring expression;
- and
- c) purifying assembled chimeric antibodies from the culture medium.
A method for producing a chimeric antibody that binds CD71, comprising:
- a) transforming a suitable host cell with at least one expression vector which comprises:
- (i) a nucleic acid molecule comprising two or more of the nucleotide sequences depicted in SEQ ID Nos. 3, 4, and 12;
- and (ii) one or more of the nucleotide sequences depicted in SEQ ID Nos. 7, 8, and 10;
- b) culturing said host cell under conditions favoring expression;
- and
- c) purifying assembled chimeric antibodies from the culture medium.
Dependencies are inferred from the claim text.