Back to results
US 8,409,570 B2Granted
Method of inducing apoptosis using anti-DR5 antibodies
AssigneeGenentech, Inc.
InventorsCamellia W. Adams, Avi J. Ashkenazi
Life of this patent
Estimating the term…
Abstract
The invention concerns anti-DR5 antibodies with improved properties, compositions comprising such antibodies, methods and means for making such antibodies, and their therapeutic use, in particular in the treatment of cancer.
Claims16
A method of inducing apoptosis comprising exposing mammalian cancer cells to an anti-DR5 antibody, or a functional fragment thereof, wherein said anti-DR5 antibody or antibody fragment comprises amino acid substitutions in the heavy and light chains of full length antibody 16E2 (SEQ ID NOS:
- 11 and 13, respectively), and has a greater affinity for DR5 than said full-length antibody 16E2, and/or exhibits a greater biological activity and/or potency than said full-length antibody 16E2, wherein said amino acid substitutions comprise:
- (a) a set of heavy chain substitutions selected from the group consisting of (i) M34L, N53Q, L102Y;
- (ii) M34L, N53Y, L102Y;
- (iii) G33A, N53Q, L102Y;
- (iv) G33A, N53Y, L102Y;
- (v) T28A, N53Q, L102Y in the amino acid sequence of SEQ ID NO:
- 11;
- and
- (b) a set of light chain substitutions selected from the group consisting of (i) Q24S, G50K, K51D, H95bY, N25S, N53E;
- (ii) Q24S, K51A, D92S, S93Y;
- and (iii) Q24S, K51A, R91A in the amino acid sequence of SEQ ID NO:
- 13.
9 more elements
Dependencies are inferred from the claim text.