Mutant antibody Fc domains and fusion proteins thereof
AssigneeMedImmune, LLC
InventorsChangshou Gao, Herren Wu
Life of this patent
Estimating the term…
Abstract
The present invention relates to a viral vector encoding for a library of antibodies or antibody fragments that are displayed on the cell membrane when expressed in a cell. The present invention provides cells comprising the viral vector nucleic acids and methods of screening the libraries for antibodies or antibody fragments with desired characteristics.
Claims12
An isolated antibody comprising a variant human IgG1 Fc region having a reduced affinity for an effector molecule wherein said human IgG1 Fc region comprises an amino acid substitution, insertion or combination thereof selected from the group consisting of:
- W277T;
- K246R/L251E/T260R;
- InR234/235;
- InV235/236;
- InR236/237;
- InR237/238;
- InV238/239;
- InN238/239;
- InL238/239;
- InE238/239;
- InG238/239;
- InS239/240;
- InG240/241;
- InE240/241;
- InG240/241/1198T;
- InL238/239/P238Q;
- InE238/239N348A;
- InS239/240/V266A;
- InR237/238/G236A, wherein the numbering is according to EU index as set forth in Kabat.
An Fc fusion protein comprising a human IgG1 Fc region having a reduced affinity for an effector molecule wherein said human IgG1 Fc region comprises an amino acid substitution, insertion or combination thereof selected from the group consisting of:
- W277T;
- K246R/L251E/T260R;
- InR234/235;
- InV235/236;
- InR236/237;
- InR237/238;
- InV238/239;
- InN238/239;
- InL238/239;
- InE238/239;
- InG238/239;
- InS239/240;
- InG240/241;
- InE240/241;
- InG240/241/1198T;
- InL238/239/P238Q;
- InE238/239N348A;
- InS239/240/V266A;
- InR237/238/G236A, wherein the numbering is according to EU index as set forth in Kabat.
Dependencies are inferred from the claim text.