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US 8,409,563 B2Granted
Purine-derived substance-producing bacterium and a method for producing a purine-derived substance
AssigneeAjinomoto Co., Inc.
InventorsTakayuki Asahara, Kiyoshi Matsuno, Yukiko Mori
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Abstract
A purine-derived substance is produced by culturing a bacterium belonging to the genus Bacillus which is able to produce purine-derived substance and has been modified so that enzymatic activity of fructose bisphosphatase is decreased, and collecting the purine-derived substance from the medium or cells.
Claims11
A method for producing a purine-derived substance, which comprises
- (A) culturing a bacterium belonging to the genus Bacillus which is able to produce a purine-derived substance in a medium, and
- (B) collecting the purine-derived substance from the bacterium or medium,
- wherein the bacterium has been modified to decrease the enzymatic activity of fructose bisphosphatase,
- wherein said fructose bisphosphatase is selected from the group consisting of:
- (i) a protein comprising the amino acid sequence of SEQ ID NO:
- 2,
- (ii) a protein comprising the amino acid sequence of SEQ ID NO:
- 2, but which includes substitutions, deletions, insertions, or additions, of one to 10 amino acid residues and has fructose bisphosphatase activity, and
- (iii) combinations thereof;
- wherein the fructose bisphosphatase activity is decreased by disrupting a gene encoding fructose bisphosphatase, or decreasing the expression of a gene encoding fructose bisphosphatase.
7 more elements
Dependencies are inferred from the claim text.