Apparatus for insulated isothermal polymerase chain reaction

In combination, an apparatus for holding a test tube and the test tube having a bottom, a middle section and an upper section in which insulated isothermal polymerase chain reaction is performed, the apparatus comprising:

• a heat insulating mount having a main body provided with a receiving space [for] receiving the bottom of the test tube, a lateral channel communicated between the receiving space and an ambient environment, and an upper channel communicated between the receiving space and the ambient environment for insertion of the test tube;
• and
• a heating member inserted into the lateral channel for stopping at and introducing heat energy into the bottom of the test tube;
• and
• a heat dissipating mount having a main body mounted on the heat insulating mount and provided with a through hole in communication with the upper channel of the heat insulating mount for insertion of the test tube, the upper and middle portion of the test tube received within the heat dissipating mount, the heat dissipating mount being adapted to transmit the heat energy at the middle and upper sections of the test tube to the ambient atmosphere while the heating member is introducing the heat energy into the bottom of the test tube.