Back to results
US 8,389,252 B2Granted
Yeast strain for production of four carbon alcohols
AssigneeButamax(Tm) Advanced Biofuels LLC
InventorRobert A. LaRossa
Life of this patent
Estimating the term…
Abstract
Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protein. Yeast strains with an engineered butanol biosynthetic pathway and a genetic modification in a gene involved in the general control response to amino acid starvation, which have increased butanol tolerance, are useful for production of butanol.
Claims13
A recombinant yeast host cell having the following characteristics:
- a) the yeast host cell produces a butanol when grown in a medium containing a carbon substrate;
- b) the yeast host cell comprises at least one genetic modification which reduces the response in the general control response to amino acid starvation, wherein the target for said genetic modification is a gene encoding the General Control Nonderepressible (Gcn) protein Gcn4p;
- and
- c) the yeast host cell comprises a recombinant biosynthetic pathway selected from the group consisting of:
- a) a 1-butanol biosynthetic pathway;
- b) a 2-butanol biosynthetic pathway;
- and
- c) an isobutanol biosynthetic pathway.
5 more elements
Dependencies are inferred from the claim text.