Modified industrial yeast strains
AssigneesThe Australian Wine Research Institute Ltd, Mauri Yeast Australia Pty Ltd
InventorsAntonio Felipe Garcia Cordente, Jan Hendrik Swiegers
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Abstract
The present invention relates to modified industrial yeast strains that show reduced hydrogen sulfide production. In one embodiment the invention provides an industrial yeast strain comprising a modification in a MET5 gene and/or a MET10 gene which results in reduced hydrogen sulfide production when compared to the corresponding industrial yeast strain without the modification. The present invention also relates to methods of manufacturing these modified industrial yeast strains and their use in the production of fermented products.
Claims14
An isolated industrial Saccharomyces cerevisiae yeast strain comprising one or more point mutations in the MET5 gene or the MET10 gene which results in reduced hydrogen sulfide production when compared to the corresponding industrial yeast strain without the modification, wherein the unmodified yeast strain is Saccharomyces cerevisiae,
- wherein the one or more point mutations in the MET5 gene result in the following amino acid substitutions in the polypeptide encoded by the MET5 gene:
- (i) P210L;
- (ii) A979T;
- (iii) G980D;
- (iv) G1115D;
- (v) E1356K;
- or
- (vi) a combination of A979T and G1115D;
- and
- wherein the one or more point mutations in the MET10 gene result in the following amino acid substitutions in the polypeptide encoded by the MET10 gene:
- (i) W59stop;
- (ii) L606F;
- (iii) E619K;
- (iv) W841 stop;
- (v) G911S;
- (vi) E929K;
- (vii) T990I
- (viii) T997I;
- or
- (ix) a combination of W841stop/G911S, E929K, T9901 or L606F and T997I.
A modified yeast strain deposited with The National Measurement Institute (NMI) Australia under the accession numbers V07/022167, V07/022168, V07/022169, V07/022170, V07/022171 or V07/022172.
Dependencies are inferred from the claim text.