Pierceable cap having piercing extensions

A pierceable cap comprising:

• a shell,
• an access port through the shell,
• a lower frangible layer disposed across the access port,
• an upper frangible layer disposed across the access port, wherein the access port is flush with a top of the shell, and
• one or more extensions between the lower frangible layer and the upper frangible layer, wherein the one or more extensions are coupled to walls of the access port by one or more coupling regions.

A pierceable cap comprising:

• a shell,
• an access port in the shell for allowing passage of at least part of a transfer device through the access port, wherein the transfer device transfers a sample specimen,
• a lower frangible layer disposed across the access port for preventing transfer of the sample specimen through the access port prior to insertion of the at least part of the transfer device, wherein the lower frangible layer comprises pre-formed scoring,
• one or more upper frangible layers disposed across the access port for preventing transfer of the sample specimen through the access port after insertion of the at least part of the transfer device through the lower frangible layer,
• one or more extensions between the lower frangible layer and the one or more upper frangible layers, and
• wherein the one or more extensions move and pierce the lower frangible layer upon application of pressure from the transfer device.

A pierceable cap comprising:

• a shell,
• an access port in the shell for allowing passage of at least part of a transfer device through the access port, wherein the transfer device transfers a sample specimen,
• a lower frangible layer disposed across the access port for preventing transfer of the sample specimen through the access port prior to insertion of the at least part of the transfer device,
• one or more upper frangible layers disposed across the access port for preventing transfer of the sample specimen through the access port after insertion of the at least part of the transfer device through the lower frangible layer, wherein the one or more upper frangible layers are flush with a top of the shell
• one or more extensions between the lower frangible layer and the one or more upper frangible layers, and
• wherein the one or more extensions move and pierce the lower frangible layer upon application of pressure from the transfer device.