Method and system for the production of cells and cell products and applications thereof

A cell culture system for the production of cells and cell derived products comprising:

• a reusable instrumentation base device incorporating hardware to support cell culture growth;
• at least one disposable cell cultureware module removably attachable to said instrumentation base device, said module including a cell growth chamber containing a semi-permeable matrix;
• a media line, for providing cell culture medium to said cell growth chamber;
• a harvest line, for transporting cells or cell derived products from said cell growth chamber and out of said cell cultureware module;
• and
• an outflow line, for transporting waste or spent media from said cell growth chamber, wherein said instrumentation base device includes a multi-channel peristaltic pump for moving fresh basal media into said at least one cultureware module, wherein said multi-channel peristaltic pump has discrete, variable output control for each channel, and wherein said at least one cultureware module further comprises a pump cassette, wherein said pump cassette is structured to hold multiple peristaltic tubing segments, and wherein said pump cassette is insertable into an interface on said instrumentation base device and allows for said tubing segments to be loaded into said multi-channel peristaltic pump in said instrumentation base device at the same time.