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US 8,383,350 B1Granted
Assay for detection of IL-10 antibodies
AssigneeMerck Sharp & Dohme Corp.
InventorsKaren Kolz, Constance M. Cullen
Life of this patent
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Abstract
Provided are assays to measure the presence and quantity of an antibody of interest in a patient's bloodstream or other biological sample. ECL and ELISA methods, and kits for such assays, as well as anti-idiotypic antibodies provided to detect levels of the antibody in biological samples, which are from, for example, animal models and human patients.
Claims16
An assay method for specifically detecting in a plasma or serum sample, a humanized 12G8 antibody that binds to mammalian IL-10 comprising
- (a) contacting and incubating the sample with a first idiotypic capture antibody selected from the group consisting of 11F12 and 13G4, which binds to the humanized 12G8 antibody but not any other antibody in the sample that binds to mammalian IL-10;
- (b) contacting the sample, and hence any bound humanized 12G8 antibody, with a second idiotypic detection antibody selected from the group consisting of 11F12 and 13G4 which binds to the humanized 12G8 antibody but not any other antibody in the sample that binds to mammalian IL-10;
- (c) measuring the level of any of the humanized 12G8 antibody bound;
- and
- d) determining the level of humanized 12G8 antibody in the plasma or serum sample using a standard titration curve.
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Dependencies are inferred from the claim text.