Production of four carbon alcohols using improved strain
AssigneeButamax(Tm) Advanced Biofuels LLC
InventorsRobert A. Larossa, Dana R. Smulski, Vasantha Nagarajan
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Abstract
Using screening of transposon random insertion mutants, genes involved in accumulation of (p)ppGpp were found to be involved in bacterial cell response to butanol. Reduced production of proteins with enzymatic activity for (p)ppGpp biosynthesis confers increased butanol tolerance. Bacterial strains with reduced (p)ppGpp accumulation and having a butanol or 2-butanone biosynthetic pathway are useful for production of butanol or 2-butanone.
Claims13
A recombinant bacterial cell producing butanol or 2-butanone said bacterial cell comprising at least one genetic modification which reduces accumulation of (p)ppGpp, wherein the at least one genetic modification is a disruption in an endogenous gene encoding SpoT or RelA or in an operon comprising an open reading frame encoding SpoT or RelA;
- a) the SpoT protein having:
- 1) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the RelA/Spot domain;
- and
- 2) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the TGS domain;
- and
- 3) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the HD domain;
- and
- b) the RelA protein having:
- a) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the RelA/SpoT domain;
- and
- b) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the TGS domain.
Dependencies are inferred from the claim text.