Method and apparatus for detecting irregularities in tissue microarrays
AssigneeGeneral Electric Company
InventorsAli Can, Michael John Gerdes, Xiaodong Tao, Musodiq Olatayo Bello, Maximilian Seel
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Abstract
The present techniques provide systems and methods for registering images of tissue spots on a tissue microarray (TMA). In studies involving multiple biomarkers being studied on the same TMA, the TMA slide is removed from the microscope, stained, and then imaged, often multiple times. The present techniques relate to validation of the registration of the acquired images of the same TMA. An automatic approach to register the images and detect registration failures as provided herein may enhance the rapid analysis of the tissues. Artifacts such as tissue folding and tissue loss are also determined automatically.
Claims26
A method for analyzing a tissue microarray, comprising the steps of:
- using a processor-based computer:
- accessing two or more images of a tissue microarray using the computer, the two or more images comprising a plurality of tissue spots;
- registering corresponding tissue spots on the two or more images using default registration parameters stored on the computer;
- determining the quality of each of the plurality of tissue spots using the computer;
- generating an output on the computer indicating the registration quality of the plurality of spots between the two or more images and the quality of the plurality of tissue spots in at least one of the two or more images, wherein generating the output comprises generating a tissue quality indicator and a registration indicator for each of the plurality of tissue spots in at least one of the two or more images;
- determining if the registration is successful based on the output;
- and
- changing the default registration parameters in response to an unsuccessful registration.
A non-transitory computer-readable medium comprising instructions for:
- accessing two or more images of a tissue microarray comprising a plurality of tissue spots;
- registering corresponding tissue spots on the two or more images using default registration parameters;
- determining the quality of each of the plurality of tissue spots;
- generating an output indicating the registration quality of the plurality of spots between the two or more images and the quality of the plurality of tissue spots in at least one of the two or more images, wherein generating the output comprises generating a tissue quality indicator and a registration indicator for each of the plurality of tissue spots in at least one of the two or more images;
- determining if the registration is successful based on the output;
- and
- changing the default registration parameters in response to an unsuccessful registration.
An image analysis system comprising:
- a processor adapted to access two or more images of a tissue microarray comprising a plurality of tissue spots, the processor adapted to run instructions for:
- registering corresponding tissue spots on the two or more images using default registration parameters;
- determining the quality of each of the plurality of tissue spots;
- generating an output indicating the registration quality of the plurality of spots between the two or more images and the quality of the plurality of tissue spots in at least one of the two or more images, wherein generating the output comprises generating an overview image in which image data representing each of the plurality of spots are shown within the overview image;
- determining if the registration is successful based on the output;
- and
- changing the default registration parameters in response to an unsuccessful registration.
A method for analyzing a tissue microarray, comprising the steps of:
- using a processor-based computer:
- accessing two or more images of a tissue microarray using the computer, the two or more images comprising a plurality of tissue spots;
- registering corresponding tissue spots on the two or more images using default registration parameters stored on the computer;
- determining the quality of each of the plurality of tissue spots using the computer;
- generating an output on the computer indicating the registration quality of the plurality of spots between the two or more images and the quality of the plurality of tissue spots in at least one of the two or more images, wherein generating the output comprises generating an overview image in which image data representing each of the plurality of spots are shown within the overview image;
- determining if the registration is successful based on the output;
- and
- changing the default registration parameters in response to an unsuccessful registration.
Dependencies are inferred from the claim text.