Method for determining carbohydrate and kit for determining carbohydrate
AssigneeNagasaki Prefectural and Municipal Univ. Corp.
InventorsTuneyuki Oku, Sadako Nakamura
Life of this patent
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Abstract
It is intended to provide a method for determining a carbohydrate which enables more accurate determination of a carbohydrate. The invention for achieving this object is directed to a method for determining a carbohydrate using a digestive enzyme, characterized in that as the digestive enzyme, an animal-derived low-molecular-weight carbohydrate digestive enzyme is used. More specifically, the invention is directed to a method for determining a carbohydrate using a digestive enzyme, characterized by comprising a first reaction step using thermostable α-amylase; a second reaction step using protease and amyloglucosidase; and a third reaction step using an animal-derived low-molecular-weight carbohydrate digestive enzyme.
Claims6
A method for determining whether a carbohydrate is a non-digestible oligosaccharide, comprising:
- (a) reacting the carbohydrate with thermostable α-amylase;
- then
- (b) reacting the reaction product of step (a) with protease and amyloglucosidase;
- then
- (c) reacting the reaction product of step (b) with a suspension of an animal-derived low-molecular-weight carbohydrate digestive enzyme, said suspension having been prepared by surgical removal of a portion of the intestine of an animal, followed by homogenization, ultrasonic disintegration and centrifugation thereof, and then
- (d) determining whether the carbohydrate is a non-digestible oligosaccharide by comparing high-performance liquid chromatography of the carbohydrate prior to step (a) with high-performance liquid chromatography of the reaction product of step (c).
A kit for determining a carbohydrate including a digestive enzyme, comprising:
- thermostable α-amylase;
- protease;
- amyloglucosidase;
- and
- a suspension of an animal-derived low molecular-weight carbohydrate digestive enzyme, said suspension having been prepared by surgical removal of a portion of the intestine of an animal, followed by homogenization, ultrasonic disintegration and centrifugation thereof.
Dependencies are inferred from the claim text.