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US 8,367,346 B2Granted
Methods for production of xylitol in microorganisms
AssigneesBiotechnology Research and Development Corporation, The United States of America, as represented by Sec. of Agriculture
InventorsPaul Taylor, Nathan Wymer, Francis Michael Racine
Life of this patent
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Abstract
The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.
Claims6
A method for screening for bacteria that cannot utilize L-arabinose comprising:
- transforming bacteria that do not have xylose isomerase activity or an araBAD operon and that have xylitol dehydrogenase activity and L-ribulokinase activity, with a nucleic acid encoding a xylose reductase, and contacting the bacteria with media comprising L-arabinose and D-xylose, wherein if the xylose reductase is a xylose-specific reductase, then the transformed bacteria cannot utilize L-arabinose and will grow on media comprising L-arabinose and D-xylose, and wherein if the xylose reductase is not a xylose-specific reductase, then the transformed bacteria can utilize L-arabinose and will not grow on media comprising L-arabinose and D-xylose.
Dependencies are inferred from the claim text.