Digital holographic microscopy
AssigneesNanyang Technological University, Ngee Ann Polytechnic
InventorsAnand Krishna Asundi, Weijuan Qu, Oi Choo Chee
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Abstract
An optical configuration for a digital holographic microscope and a method for digital holographic microscopy are presented. In one embodiment, digital off-axis holograms are obtained using a cube beam splitter (110) to both split and combine a diverging spherical wavefront emerging from a microscope objective (108). When a plane numerical reference wavefront is used for the reconstruction of the recorded digital hologram, the phase curvature introduced by the microscope objective (108) together with the illuminating wave to the object wave can be physically compensated.
Claims23
A digital holographic microscope comprising:
- a microscope objective configured to receive radiation from an object and to provide a beam of radiation having a phase curvature,
- a beam splitter configured to split the beam of radiation into a first portion and a second portion, introduce an optical path difference between the first and second portions, and combine the first and second portions to produce an interference pattern that is substantially free of the phase curvature, and
- a transducer configured to generate digital hologram signals indicative of the interference pattern.
A method of digital holographic microscopy comprising:
- providing a beam of radiation from radiation received from an object, the beam of radiation having a phase curvature,
- splitting the beam of radiation into a first portion and a second portion,
- introducing an optical path difference between the first and second portions,
- combining the first and second portions to produce an interference pattern that is substantially free of the phase curvature, and
- generating digital hologram signals indicative of the interference pattern.
Dependencies are inferred from the claim text.