Detection and typing of bacterial strains
AssigneeDupont Nutrition Biosciences Aps
InventorsW. Michael Russell, Rodolphe Barrangou, Philippe Horvath
Life of this patent
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Abstract
Methods for the detection and typing of bacterial strains from food products and dietary supplements, environmental samples, in vivo/in vitro samples, and for studying the natural diversity of the species are disclosed. Potential applications also include product development and/or detection and differentiation of new bacterial strains.
Claims8
A method for typing a Lactobacillus species having a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) region, comprising:
- a) obtaining a sample comprising said Lactobacillus;
- b) amplifying a region of DNA comprising said CRISPR region or a fragment thereof in said sample to create amplified DNA, ;
- and
- c) typing a Lactobacillus species having a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) region.
A method for typing a Lactobacillus species having a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) region, comprising:
- a) obtaining a sample comprising said Lactobacillus;
- b) amplifying a region of DNA comprising said CRISPR region or a fragment thereof in said sample to create amplified DNA, the CRISPR region including at least one CRISPR repeat region or CRISPR spacer region or a portion thereof;
- and
- c) typing a Lactobacillus species having a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) region.
Dependencies are inferred from the claim text.