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US 8,361,709 B2Granted
Method of producing tooth, set of teeth, and method of producing tissue
AssigneeOrgan Technologies, Inc.
InventorsTakashi Tsuji, Kazuhisa Nakao
Life of this patent
Estimating the term…
Abstract
A first cell mass substantially containing only either one of mesenchymal cells or epithelial cells and a second cell mass substantially containing only the other one of the cells are positioned in contact with each other inside a support carrier which can maintain a condition of cell contact; and cultured to obtain a tooth having a specific cell placement. Preferably, after the culturing, the support carrier having both cell masses is cultured with kidney cells.
Claims23
A method of producing a tooth, comprising:
- positioning a first cell mass substantially containing only either one of mesenchymal cells or epithelial cells, wherein at least one of the mesenchymal cells or the epithelial cells is derived from a tooth germ, and a second cell mass substantially containing only the other one of the mesenchymal cells or the epithelial cells, inside a support carrier, such that the first and second cell masses are in a state of close contact with each other inside the support carrier without mixing between the cell masses, wherein the mesenchymal cells or the epithelial cells are in close contact with each other in the corresponding cell mass;
- positioning the first cell mass inside the support carrier such that the second cell mass makes a depression in the first cell mass such that the first cell mass substantially envelops the second cell mass when the second cell mass is inserted into the support carrier;
- and
- culturing the first and the second cell masses inside the support carrier until the tooth is formed, while maintaining the state of close contact between the cell masses.
1 more element
A method of producing a tooth with periodontal tissue comprising:
- positioning a first cell mass substantially containing only either one of mesenchymal cells or epithelial cells, wherein at least one of the mesenchymal cells or the epithelial cells is derived from a tooth germ, and a second cell mass substantially containing only the other one of the mesenchymal cells or the epithelial cells, inside a support carrier, such that the first and the second cell masses are in a state of close contact with each other inside the support carrier without mixing between the cell masses, wherein the mesenchymal cells or the epithelial cells are in close contact with each other in the corresponding cell mass;
- positioning the first cell mass inside the support carrier such that the second cell mass makes a depression in the first cell mass such that the first cell mass substantially envelops the second cell mass when the second cell mass is inserted into the support carrier;
- culturing the first and the second cell masses inside the support carrier until the tooth is formed, while maintaining the state of close contact between the cell masses;
- and
- continuing the culturing until the periodontal tissue is formed.
2 more elements
A method of producing periodontal tissue, comprising:
- positioning a first cell mass substantially containing only either one of mesenchymal cells or epithelial cells, wherein at least one of the mesenchymal cells or the epithelial cells is derived from a tooth germ, and a second cell mass substantially containing only the other one of the mesenchymal cells or the epithelial cells, inside a support carrier, such that the first and second cell masses are in a state of close contact with each other inside the support carrier without mixing between the cell masses, wherein the mesenchymal cells or the epithelial cells are in close contact with each other in the corresponding cell mass;
- positioning the first cell mass inside the support carrier such that the second cell mass makes a depression in the first cell mass such that the first cell mass substantially envelops the second cell mass when the second cell mass is inserted into the support carrier;
- culturing the first and the second cell masses inside the support carrier until a tooth and periodontal tissue contiguous to the tooth are obtained, while maintaining the state of close contact between the cell masses;
- and
- isolating the periodontal tissue obtained by the culturing.
2 more elements
A set of teeth, obtained by
- positioning a first cell mass substantially containing only either one of mesenchymal cells or epithelial cells, wherein at least one of the mesenchymal cells or the epithelial cells is derived from a tooth germ, and a second cell mass substantially containing only the other one of the mesenchymal cells or the epithelial cells, inside a support carrier, such that the first and the second cell masses are in a state of close contact with each other inside the support carrier without mixing between the cell masses, wherein the mesenchymal cells or the epithelial cells are in close contact with each other in the corresponding cell mass;
- positioning the first cell mass inside the support carrier such that the second cell mass makes a depression in the first cell mass such that the first cell mass substantially envelops the second cell mass when the second cell mass is inserted into the support carrier;
- and
- culturing the first and the second cell masses inside the support carrier until the set of teeth is formed, while maintaining the state of close contact between the cell masses.
1 more element
A method of producing a tissue that is constructed by an interaction between mesenchymal cells and epithelial cells, the method comprising:
- positioning a first cell mass substantially containing only either one of mesenchymal cells or epithelial cells and a second cell mass substantially containing only the other one of the mesenchymal cells or the epithelial cells, inside a support carrier, such that the first and the second cell masses are in a state of close contact with each other inside the support carrier without mixing between the cell masses, wherein the mesenchymal cells or the epithelial cells are in close contact with each other in the corresponding cell mass;
- positioning the first cell mass inside the support carrier such that the second cell mass makes a depression in the first cell mass such that the first cell mass substantially envelops the second cell mass when the second cell mass is inserted into the support carrier;
- and
- culturing the first and the second cell masses inside the support carrier until the tissue is formed, while maintaining the state of close contact between the cell masses.
1 more element
Dependencies are inferred from the claim text.