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US 8,357,652 B2Granted
Anti-tumor fibrillar human serum albumin methods and compositions
AssigneeAcademia Sinica
InventorsShu-Mei Liang, Chi-Ming Liang
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Abstract
Fibrillar human serum albumin was shown to be effective in the treatment of various types of cancers. Methods and compositions are disclosed for using fibrillar human serum albumin as a medicament to treat subjects having cancer.
Claims2
A method comprising:
- administering to a subject having cancer selected from breast cancer, ovarian or cervical cancer, prostate cancer, lung cancer, and liver cancer, a therapeutically effective amount of fibrillar human serum albumin, wherein the fibrillar human serum albumin is produced by a method comprising:
- dissolving human serum albumin in a suitable detergent;
- sonicating the human serum albumin;
- applying the sonicated human serum albumin to a gel filtration column with a separation range above 70 kDa molecular weight;
- so as to promote column-induced fibrillar protein formation and to separate globular protein from fibrillar protein;
- eluting the fibrillar human serum albumin from the column with a buffer containing a low concentration of detergent;
- collecting eluent containing fibrillar human serum albumin from the column, and
- dialyzing the eluant to remove the detergent.
5 more elements
Dependencies are inferred from the claim text.