Method of purifying influenza virus and removing MDCK cell DNA contaminants
AssigneeMemimmune, LLC
InventorsJonathan Liu, Richard Schwartz, Mark Thompson, Luis Jorge Camilo Maranga, Simon Sheng-Tsiung Hsu, Mridul Ghosh, Ajit Subramanian
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.
Claims14
A method for removing a Madin-Darby Canine Kidney (MDCK) cell DNA contaminant from an influenza virus preparation, comprising:
- (a) passing an influenza virus preparation from an influenza virus infected MDCK cell culture over an affinity chromatography media;
- wherein the influenza virus is retained on the media and a DNA contaminant is not retained on the media;
- (b) after (a), passing a composition comprising a non-specific endonuclease over the chromatography media;
- (c) after (b), washing the chromatography media;
- and
- (d) after (c), eluting the influenza virus from the chromatography media.
Dependencies are inferred from the claim text.