Multi plasmid system for the production of influenza virus
AssigneeMedImmune, LLC
InventorsBin Lu, Hong Jin
Life of this patent
Estimating the term…
Abstract
Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics.
Claims9
A reassortant influenza A virus comprising an altered H3 HA protein that is replication enhanced relative to the virus without the altered H3 HA protein, wherein the altered H3 HA protein comprises substitutions at positions 186 and 196;
- wherein the substitutions change the amino acids at positions 186 and 196 to valine and threonine, respectively.
Dependencies are inferred from the claim text.