Glucose dehydrogenase/cytochrome fusion protein
AssigneesARKRAY, Inc., Ultizyme International Ltd.
InventorKoji Sode
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Abstract
A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.
Claims7
A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome, wherein the cytochrome has been fused to the C-terminal side of PQQGDH, and wherein the PQQGDH is either (a) or (b):
- (a) a protein comprising an amino acid sequence represented by SEQ ID NO:
- 2;
- (b) a protein comprising an amino acid sequence in which one amino acid residue has been deleted, substituted or added in the amino acid sequence (a) and having a glucose dehydrogenase activity and an electron transfer ability, and wherein the cytochrome is cytochrome c or cytochrome B562.
Dependencies are inferred from the claim text.