Methods and systems for chemoautotrophic production of organic compounds
AssigneeGinkgo Bioworks, Inc.
InventorsCurt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
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Abstract
The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.
Claims19
An engineered cell for producing a carbon-based product, comprising:
- an at least partially engineered energy conversion pathway having a recombinant formate dehydrogenase or a recombinant sulfide-quinone oxidoreductase introduced into a host cell, wherein
- the recombinant formate dehydrogenase is encoded by SEQ ID NO:1-4 or a homolog thereof having at least 80% sequence identity thereto, or comprises any one of SEQ ID NOs:5-8 or a homolog thereof having at least 80% sequence identity thereto,
- the recombinant sulfide-quinone oxidoreductase is encoded by any one of SEQ ID NOs:9-16 or a homolog thereof having at least 80% sequence identity thereto, and
- said energy conversion pathway is capable of using energy from oxidation to produce a reduced cofactor;
- a carbon fixation pathway that is capable of converting inorganic carbon to a carbon-based central metabolite using energy from the reduced cofactor;
- and
- optionally, a carbon product biosynthetic pathway that is capable of converting the carbon-based central metabolite into a carbon-based product of interest.
Dependencies are inferred from the claim text.