Back to results
US 8,349,570 B2Granted
Enhancing endotoxin detection
AssigneeBioDtech, Inc.
InventorsMichael G. Pepe, Milton Keith Champion
Life of this patent
Estimating the term…
Abstract
Provided herein are methods for detecting gram negative bacteria or lipopolysaccharide in a sample. Kits for detecting gram negative bacteria or lipopolysaccharide in a sample are provided.
Claims6
A method for detecting gram negative bacteria or lipopolysaccharide in a sample consisting of the steps of:
- (a) contacting the sample with an active acidic protease under conditions that result in degradation of the proteins in the sample;
- (b) further contacting the sample with an amebocyte lysate after step (a), wherein the sample comprises the degraded proteins;
- and
- (c) determining whether a gelation reaction occurs in the amebocyte lysate, a gelation reaction indicating the sample of step (b) contains gram negative bacteria or lipopolysaccharide.
1 more element
A method for detecting gram negative bacteria or lipopolysaccharide in a sample consisting of the steps of:
- (a) contacting the sample with an active acidic protease under conditions that result in degradation of the proteins in the sample;
- (b) inactivating the acidic protease after step (a);
- (c) further contacting the sample with an amebocyte lysate after step (b), wherein the sample comprises the degraded proteins;
- and
- (d) determining whether a gelation reaction occurs in the amebocyte lysate, a gelation reaction indicating the sample of step (c) contains gram negative bacteria or lipopolysaccharide.
2 more elements
A method for detecting gram negative bacteria or lipopolysaccharide in a sample consisting of the steps of:
- (a) contacting the sample with an active acidic protease under conditions that result in degradation of the proteins in the sample;
- (b) diluting the sample;
- (c) inactivating the acidic protease after step (a);
- (d) further contacting the sample with an amebocyte lysate after step (c), wherein the sample comprises the degraded proteins;
- and
- (e) determining whether a gelation reaction occurs in the amebocyte lysate, a gelation reaction indicating the sample of step (d) contains gram negative bacteria or lipopolysaccharide.
3 more elements
Dependencies are inferred from the claim text.