US 8,344,205 B2 · Plants with increased yield

Abstract

This invention relates generally to plant cells and/or plants with increased yield as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of polypeptides associated with the intermediate phosphoribosylpyrophosphate (PRPP) in plants. In particular, this invention relates to plant cells and/or plants with increased yield as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of phosphoribosyl pyrophosphate synthases (PRPP synthetase, PRS) The invention also deals with methods of producing and screening for and breeding such plant cells and/or plants.

Claims17

Dependent claims

A method for producing a transgenic plant cell, plant, or part thereof with increased yield as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof comprising:

a) introducing a nucleic acid sequence encoding a phosphoribosyl pyrophosphate synthase into a plant cell, plant, or part thereof, such that the phosphoribosyl pyrophosphate synthase is expressed;
and
b) selecting a plant cell, plant, or part thereof with increased yield as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof.

A transformed host cell comprising at least one nucleic acid molecule comprising:

(a) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, and 63;
(b) a nucleic acid molecule selected from the group consisting of SEQ ID NO:
1, 3, and 12;
(c) a nucleic acid molecule that has at least 75% sequence identity with the nucleic acid molecule of SEQ ID NO:
1, 3, or 12 and that confers an increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof;
(d) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence having at least 75% sequence identity with the amino acid sequence encoded by the nucleic acid molecule of (a) or (b), wherein said polypeptide has the activity of the polypeptide of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, or 63 and confers an increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof;
(e) a nucleic acid molecule which hybridizes with the nucleic acid molecule of (a) or (b) under stringent hybridization conditions and confers an increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof, wherein said stringent hybridization conditions are selected from the group consisting of:
i) hybridization in 6×SSC at approximately 45° C.;
ii) hybridization in 4×SSC at 65° C.;
and
iii) hybridization in 50% formamide, 4×SSC at 42° C.;
(f) a nucleic acid molecule encoding a polypeptide which can be isolated with the aid of a monoclonal or polyclonal antibody made against a polypeptide encoded by one of the nucleic acid molecules of (a) or (b) and having the activity of a phosphoribosyl pyrophosphate synthase, wherein said nucleic acid molecule comprises a polynucleotide selected from the group consisting of SEQ ID NO:
1, 3, 12, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, and 50;
(g) a nucleic acid molecule encoding a polypeptide comprising a consensus sequence or one or more polypeptide motifs selected from the group consisting of SEQ ID NO:
7, 8, 9, 10, 11, 14, 15, 16, 17, 18, 64, 65, 66, 67, 68, 69, 70, 71, 72, and 73 and having the activity of a phosphoribosyl pyrophosphate synthase, wherein said phosphoribosyl pyrophosphate synthase comprises a polypeptide selected from the group consisting of SEQ ID NO:
NO:
2, 4, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 64, 65, 66, 67, 68, 69, 70, 71, 72, and 73;
(h) a nucleic acid molecule that encodes a polypeptide having the activity of a phosphoribosyl pyrophosphate synthase and confers increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof, wherein said phosphoribosyl pyrophosphate synthase comprises a protein selected from the group consisting of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, and 63;
(i) a nucleic acid molecule which comprises a polynucleotide which is obtained by amplifying a cDNA library or a genomic library using primers selected from the group consisting of SEQ ID NOs:
5 and 6, wherein said polynucleotide encodes a polypeptide having the activity of a phosphoribosyl pyrophosphate synthase, and comprises a polynucleotide selected from the group consisting of SEQ ID NO:
1, 3, 12, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, and 50;
or
(j) a nucleic acid molecule obtained by screening a suitable nucleic acid library under stringent hybridization conditions with a probe comprising a complementary sequence of a nucleic acid molecule of (a) or (b) or with a fragment thereof, having at least 15 nt of a nucleic acid molecule complementary to a nucleic acid molecule sequence characterized in (a) to (d) and encoding a polypeptide having the activity of a phosphoribosyl pyrophosphate synthase comprising a polypeptide selected from the group consisting of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, and 63, wherein said stringent hybridization conditions are selected from the group consisting of:
i) hybridization in 6×SSC at approximately 45° C.;
ii) hybridization in 4×SSC at 65° C.;
and
iii) hybridization in 50% formamide, 4×SSC at 42° C.

28 more elements

A method for selecting a plant or plant cell with an increased yield as compared to a corresponding non-transformed wild type plant or plant cell comprising utilizing a nucleic acid molecule as a marker for selecting a plant or plant cell with an increased yield as compared to a corresponding non-transformed wild type plant or plant cell, wherein the nucleic acid molecule comprises:

(a) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, and 63;
(b) a nucleic acid molecule selected from the group consisting of SEQ ID NO:
1, 3, and 12;
(c) a nucleic acid molecule that has at least 75% sequence identity with the nucleic acid molecule of SEQ ID NO:
1, 3, or 12 and that confers an increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof;
(d) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence having at least 75% sequence identity with the amino acid sequence encoded by the nucleic acid molecule of (a) or (b), wherein said polypeptide has the activity of the polypeptide of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, or 63 and confers an increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof;
(e) a nucleic acid molecule which hybridizes with the nucleic acid molecule of (a) or (b) under stringent hybridization conditions and confers an increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof, wherein said stringent hybridization conditions are selected from the group consisting of:
i) hybridization in 6×SSC at approximately 45° C.;
ii) hybridization in 4×SSC at 65° C.;
and
iii) hybridization in 50% formamide, 4×SSC at 42° C.;
(f) a nucleic acid molecule encoding a polypeptide which can be isolated with the aid of a monoclonal or polyclonal antibody made against a polypeptide encoded by one of the nucleic acid molecules of (a) or (b) and having the activity of a phosphoribosyl pyrophosphate synthase, wherein said nucleic acid molecule comprises a polynucleotide selected from the group consisting of SEQ ID NO:
1, 3, 12, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, and 50;
(g) a nucleic acid molecule encoding a polypeptide comprising a consensus sequence or one or more polypeptide motifs selected from the group consisting of SEQ ID NO:
7, 8, 9, 10, 11, 14, 15, 16, 17, 18, 64, 65, 66, 67, 68, 69, 70, 71, 72, and 73 and having the activity of a phosphoribosyl pyrophosphate synthase, wherein said phosphoribosyl pyrophosphate synthase comprises a polypeptide selected from the group consisting of SEQ ID NO:
2, 4, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 64, 65, 66, 67, 68, 69, 70, 71, 72, and 73;
(h) a nucleic acid molecule that encodes a polypeptide having the activity of a phosphoribosyl pyrophosphate synthase and confers increased yield to a plant cell, plant, or part thereof as compared to a corresponding non-transformed wild type plant cell, plant, or part thereof, wherein said phosphoribosyl pyrophosphate synthase comprises a protein selected from the group consisting of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, and 63;
(i) a nucleic acid molecule which comprises a polynucleotide which is obtained by amplifying a cDNA library or a genomic library using primers selected from the group consisting of SEQ ID NO:
5 and 6, wherein said polynucleotide encodes a polypeptide having the activity of a phosphoribosyl pyrophosphate synthase, and comprises a polynucleotide selected from the group consisting of SEQ ID NO:
1, 3, 12, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, and 50;
or
(j) a nucleic acid molecule obtained by screening a suitable nucleic acid library under stringent hybridization conditions with a probe comprising a complementary sequence of a nucleic acid molecule of (a) or (b) or with a fragment thereof, having at least 15 nt of a nucleic acid molecule complementary to a nucleic acid molecule sequence characterized in (a) to (d) and encoding a polypeptide having the activity of a phosphoribosyl pyrophosphate synthase comprising a polypeptide selected from the group consisting of SEQ ID NO:
2, 4, 13, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, and 63.

23 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/444,728Filed 12 Oct 2007

Priority

EP 0612222513 Oct 2006

Published

US 2010/0037350 A111 Feb 2010

Granted

1 Jan 2013

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 15/8247
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified lipid metabolism, e.g. seed…
C12N 9/1235
Transferring phosphorus containing groups, e.g. kinases: diphosphotransferases
C12N 15/8261
Phenotypically and genetically modified plants via recombinant DNA technology: with agronomic traits, e.g. crop yield
Y02A 40/146
In agriculture: genetically Modified [GMO] plants, e.g. transgenic plants

Plants with increased yield

Life of this patent

Plants with increased yield

Life of this patent

Abstract

Claims17