Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them

An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having glucoamylase activity, selected from the group consisting of:

• (a) a nucleic acid encoding a polypeptide having glucoamylase activity comprising a nucleic acid sequence having at least 95% sequence identity to SEQ ID NO:25;
• (b) a nucleic acid encoding a polypeptide having glucoamylase activity comprising a nucleic acid sequence having at least 95% sequence identity to SEQ ID NO:25, or a fragment thereof, wherein the fragment encodes a polypeptide having glucoamylase activity;
• (c) a nucleic acid encoding a polypeptide having glucoamylase activity that hybridizes under at least high stringency conditions to the full length nucleic acid sequence of SEQ ID NO:25, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing using 6×SSC, 0.5% SDS at 50° C.;
• (d) a nucleic acid sequence encoding a polypeptide having glucoamylase activity comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:26;
• (e) a nucleic acid sequence encoding a polypeptide having glucoamylase activity comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:26, or a fragment thereof, wherein the fragment has glucoamylase activity;
• (f) the nucleic acid of (a), (b), (c), (d), or (e) encoding a polypeptide having glucoamylase activity but lacking a signal sequence or a carbohydrate binding module;
• (g) the nucleic acid of (a), (b), (c), (d), (e) or (f) encoding a polypeptide having glucoamylase activity, and further comprising a heterologous sequence;
• (h) the nucleic acid sequence of (g), wherein the heterologous sequence comprises a sequence encoding a heterologous signal sequence, carbohydrate binding module, catalytic domain (CD), or a combination thereof, or the heterologous signal sequence, carbohydrate binding module, or catalytic domain (CD) is derived from another amylase enzyme, or a non-amylase enzyme;
• or
• (i) a nucleic acid sequence fully complementary to (a), (b), (c), (d), (e), (f), (g), or (h).