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AssigneeKorea Research Institute of Bioscience and Biotechnology
InventorsChul-Ho Kim, Jeong-Woo Seo, Baek Rock Oh, Sun-Yeon Heo, Mi Young Seo, Min Ho Choi, Jin-Oh Baek, Pil-Soo Seo
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Provided is a method of producing 1,3-propanediol by culturing a recombinant strain in which the glycerol oxidative pathway had been blocked, and more particularly a method of producing 1,3-propanediol by two-step culture of a recombinant strain in which the oxidative pathway that produces byproducts in the glycerol metabolic pathway had been blocked. When the recombinant strain in which the glycerol oxidative pathway that produces byproducts had been blocked is cultured in two steps, 1,3-propanediol can be produced with improved yield without producing products that result in an increase in purification costs.
A microbial mutant which has the ability to produce 1,3-propanediol using glycerol as a carbon source, in which the Klebsiella pneumoniae strain having Korean Collection for Type Cultures (KCTC) deposit number KCTC11419BP is transformed with a vector comprising a 1,3-propanediol oxidoreductase-encoding gene or in which the 1,3-propanediol oxidoreductase-encoding gene is inserted into the chromosome of the Klebsiella pneumoniae strain having KCTC deposit number KCTC11419BP.
A microbial mutant which has the ability to produce 1,3-propanediol using glycerol as a carbon source, in which the Klebsiella pneumoniae strain having KCTC deposit number KCTC11419BP is transformed with a vector comprising a 1,3-propanediol oxidoreductase-encoding gene and a glycerol dehydratase reactivation factor-encoding gene or in which the 1,3-propanediol oxidoreductase-encoding gene and the glycerol dehydratase reactivation factor-encoding gene are inserted into the chromosome of the Klebsiella pneumoniae strain having KCTC deposit number KCTC11419BP.
A microbial mutant which has the ability to produce 1,3-propanediol using glycerol as a carbon source, in which the Klebsiella pneumoniae strain having KCTC deposit number KCTC11420BP is transformed with a vector comprising a 1,3-propanediol oxidoreductase-encoding gene or in which the 1,3-propanediol oxidoreductase-encoding gene is inserted into the chromosome of the Klebsiella pneumoniae strain having KCTC deposit number KCTC11420BP.
A microbial mutant which has the ability to produce 1,3-propanediol using glycerol as a carbon source, in which the Klebsiella pneumoniae strain having KCTC deposit number KCTC11420BP is transformed with a vector comprising a 1,3-propanediol oxidoreductase-encoding gene and a glycerol dehydratase reactivation factor-encoding gene or in which the 1,3-propanediol oxidoreductase-encoding gene and the glycerol dehydratase reactivation factor-encoding gene are inserted into the chromosome of the Klebsiella pneumoniae strain having KCTC deposit number KCTC11420BP.
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