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US 8,334,138 B2Granted
Methods and compositions for feeder-free pluripotent stem cell media containing human serum
AssigneeViaCyte, Inc.
InventorsAllan J Robins, Thomas C Schulz
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Abstract
The present invention provides compositions and methods for the culture and maintenance of pluripotent stem cells. More particularly, the present invention provides for compositions and methods for culturing, maintaining, growing and stabilizing primate pluripotent stem cells in a feeder-free defined media further comprising human serum, or a soluble attachment component of the human serum, for promoting cell attachment.
Claims19
A tissue culture composition consisting of:
- a. a primate pluripotent stem cell;
- b. a defined culture medium that supports proliferation of the primate pluripotent stem cell in an undifferentiated state;
- and
- c. sufficient human serum to promote attachment of the primate pluripotent stem cell to a tissue culture vessel, wherein the tissue culture vessel is uncoated and free of matrix,
- wherein the composition does not comprise conditioned media and is essentially free of feeder cells.
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A method of culturing a primate pluripotent stem cell comprising:
- a. plating the primate pluripotent stem cell in a unconditioned and feeder-free combination consisting of:
- i. a defined culture media medium that supports proliferation of the primate pluripotent stem cell in a undifferentiated state;
- and
- ii. sufficient human serum to promote attachment of the primate pluripotent stem cell to a tissue culture vessel, wherein the tissue culture vessel is uncoated and is free of matrix,
- b. incubating the primate pluripotent stem cell plated in step a, thereby culturing the primate pluripotent stem cell.
2 more elements
Dependencies are inferred from the claim text.