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US 8,333,975 B2Granted
Influenza hemagglutinin and neuraminidase variants
AssigneeMedImmune, LLC
InventorsChin-Fen Yang, George Kemble, Chongguang Liu
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Abstract
Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided.
Claims20
A 6:2 reassortant influenza A virus, wherein said virus comprises 6 internal genome segments from one or more donor viruses and 2 surface antigen genome segments, wherein the surface antigen genome segments encode an HA and a NA polypeptide, wherein the HA polypeptide comprises an amino acid sequence selected from SEQ ID NO:
- 89 and SEQ ID NO:
- 91.
A method for producing an influenza A virus in cell culture, the method comprising:
- i) introducing into a population of host cells, which population of host cells is capable of supporting replication of influenza virus, a plurality of vectors comprising nucleotide sequences corresponding to:
- (a) at least 6 internal genome segments of a first influenza strain;
- and, at least one genome segment encoding an HA surface antigen polypeptide wherein the surface antigen polypeptide comprises an amino acid sequence selected from SEQ ID NO:
- 89 and SEQ ID NO:
- 91;
- or
- (b) at least 6 internal genome segments of a first influenza strain, which influenza strain comprises one or more phenotypic attributes selected from the group consisting of:
- attenuated, cold adapted and temperature sensitive;
- and, at least one genome segment encoding an HA surface antigen wherein the surface antigen polypeptide comprises an amino acid sequence selected from SEQ ID NO:
- 89 and SEQ ID NO:
- 91;
- ii) culturing the population of host cells at a temperature less than or equal to 35° C.;
- and,
- iii) recovering an influenza virus.
11 more elements
Dependencies are inferred from the claim text.